Translational_Unit

Part:BBa_K3288051:Design

Designed by: Wooseok Kim   Group: iGEM19_SIS_Korea   (2019-10-17)


pOXB20-RBS(Dawn)-signal peptide (NSP4) fused MHETase(18-604;W397A)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1338
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1338
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1338
    Illegal BglII site found at 1
    Illegal BamHI site found at 795
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1338
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1338
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 69
    Illegal BsaI site found at 259
    Illegal BsaI site found at 1063


Design Notes

...


Source

...

References